常用的熒光染料
激光 | 名稱 | 激發(fā)光波長(nm) | 發(fā)射光波峰(nm) | 相對強度 (1= 最低,5 = 最高) | 濾光片 |
Violet Laser | eFluor? 450 | 405 | 450 | 2 | 450/50 |
eFluor? 605NC | 405 | 605 | 2 | 605/40 | |
eVolve?655 | 405 | 655 | 3 | 660/40 | |
Blue Laser | FITC (Fluorescein Isothiocyanate) | 488 | 518 | 3 | 530/30 |
Alexa Fluor? 488 | 488 | 519 | 3 | 530/30 | |
R-PE (R-Phycoerythrin) | 488 | 578 | 5 | 575/26 | |
PE-eFluor? 610 | 488 | 607 | 3 to 4 | 610/20 | |
PE-Cyanine 5 | 488 | 667 | 4 | 670/14 | |
PE-Cyanine 5.5 | 488 | 695 | 3 to 4 | 695/40 | |
PerCP-Cyanine 5.5 | 488 | 695 | 3 | 695/40 | |
PerCP-eFluor? 710 | 488 | 710 | 4 | 710/50 | |
PE-Cyanine 7 | 488 | 785 | 3 | 780/60 | |
7-AAD | 488 | 647 | -- | 670/14 | |
Propidium Iodide (PI) | 488 | 617 | -- | 575/26 | |
Green Laser | Alexa Fluor? 532 | 532 | 561 | 3 | 560/14 |
R-PE (R-Phycoerythrin) | 532 - 561 | 578 | 5 | 575/26 | |
PE-eFluor? 610 | 532 - 561 | 607 | 3 to 4 | 610/20 | |
PE-Cyanine 5 | 532 - 561 | 667 | 4 | 670/14 | |
PE-Cyanine 5.5 | 532 - 561 | 695 | 3 to 4 | 695/40 | |
PE-Cyanine 7 | 532 - 561 | 785 | 3 | 780/60 | |
Red Laser | APC (Allophycocyanin) | 633 | 660 | 4 | 660/20 |
eFluor? 660 | 633 | 660 | 4 | 660/20 | |
Alexa Fluor? 647 | 633 | 668 | 4 | 660/20 | |
Cy5 | 633 | 670 | 3 | 660/20 | |
Alexa Fluor? 700 | 633 | 723 | 2 | 大部分儀器要求 一個 685 LP 濾光鏡 和710/20 濾光片一起使用 | |
APC-eFluor? 780 | 633 | 780 | 2 | 780/60
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